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SDS-PAGE



Gel
Schopnost separace
15%
15-45 kDa
12.5%
15-60 kDa
10%
18-75 kDa
7.5%
30-120 kDa
5%
60-212 kDa



Vypocet slozeni x% separacniho gelu:


Kapacita bunek:


Po naliti gelu (separacni gel polymeruj 60', zavadeci gel asi 30'):


Nejbeznejsi koncentrace gelu:

Separacni gel:

10%
15%
Akrylamid/Bis-akrylamid
2.5 ml
3.75 ml
Pufr pro separacni gel (bez SDS)
1.87 ml
1.87 ml
SDS (10%)
75 ul
75 ul
H2O
3 ml
1.75 ml
10% Persulfat amonny
45 ul
45 ul
TEMED
5 ul
5 ul
Celkovy obem
7.5 ml
7.5 ml


Zavadeci gel:

Akrylamid/Bis-akrylamid
0.5 ml
Pufr pro separacni gel (bez SDS)
0.93 ml
SDS (10%)
37.5 ul
H2O
2.25 ml
10% Persulfat amonny
45 ul
TEMED
5 ul
Celkovy obem
3.75 ml